T-blocker: a simple and robust probe-free quantitative PCR assay to detect somatic mutations down to 0.1% frequency
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چکیده
منابع مشابه
A quantitative competitor PCR assay to detect genetically modified roundup-ready soybeans in commercially sold foods in Iran
Due to ever-increasing global diffusion and related socio-economic implications, the detectionof genetically modified organisms (GMOs) is very important. In this study, we design a plasmidcontaining two genes in mutated form as construct-specific (cp4 epsps) and event-specific(pd35S). It is applied for quantitative-competitor (QC) PCR as a simple and reliable method forthe detection of GM food....
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7 1. Department of Pathology, New York University School of Medicine, New York, NY, United 8 States 9 2. Department of Immunology and Applied Biotechnology, University of Rome “Tor Vergata”, 10 Rome, Italy 11 3. “Chantal Biya” International Reference Center, Sequencing Unit, Yaounde, Cameroon 12 4. Faculty of Sciences, University of Dschang, Dschang, Cameroon 13 5. Serology Unit, Medical Diagno...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2018
ISSN: 0736-6205,1940-9818
DOI: 10.2144/btn-2018-0111